London - Angelique Kerber's defence of her Wimbledon title came to an early end after being knocked out in the second round by lucky loser Lauren Davis 2-6, 6-2, 6-1 on Thursday.
The 31-year-old German, who came into the tournament in form having reached the final at Eastbourne, made her earliest exit since losing in the same round in 2013.
Davis has now equalled her best previous performance at Wimbledon in reaching the third round where she will play Spanish 30th seed Carla Suarez Navarro for a place in the last 16.
"This mens everything as it is what I work for," said Davis.
"I was a bit nervous and I slid and hurt my foot in the first set.
"I am so happy to win, it is almost surreal."
Davis, who had strapping on her leg and shoulder, had been ranked outside the top 250 earlier this year but has risen back into the top 100.
"Yeah its been a tough journey and a process of learning and growing as a player and person," she said.
"The drop in ranking has made this win even more fulfilling."
Davis said of course doubts had set in but deep down she always had belief she could succeed.
"I believe in my ability," she said.
"For me in the match the turning point was when I was 3-2 up in the second set and I said to myself you belong here, you can do this."
Wimbledon results on Thursday (x denotes seeding):
Men
2nd round
Sam Querrey (USA) bt Andrey Rublev (RUS) 6-3, 6-2, 6-3
John Millman (AUS) bt Laslo Djere (SRB x31) 6-3, 6-2, 6-1
Tennys Sandgren (USA) bt Gilles Simon (FRA x20) 6-2, 6-3, 4-6, 3-6, 8-6
Fabio Fognini (ITA x12) bt Márton Fucsovics (HUN) 6-7 (6/8), 6-4, 7-6 (7/3), 2-6, 6-3
João Sousa (POR) bt Marin Cilic (CRO x13) 6-4, 6-4, 6-4
Dan Evans (GBR) bt Nikoloz Basilashvili (GEO x18) 6-3, 6-2, 7-6 (7/2)
Jo-Wilfried Tsonga (FRA) bt Ricardas Berankis (LTU) 7-6 (7/4), 6-3, 6-3
Rafael Nadal (ESP x3) bt Nick Kyrgios (AUS) 6-3, 3-6, 7-6 (7/5), 7-6 (7/3)
Kei Nishikori (JPN x8) bt Cameron Norrie (GBR) 6-4, 6-4, 6-0
Steve Johnson (USA) bt Alex De Minaur (AUS x25) 3-6, 7-6 (7/4), 6-3, 3-6, 6-3
Jan-Lennard Struff (GER x33) bt Taylor Fritz (USA) 6-4, 6-3, 5-7, 7-6 (7/2)
Mikhail Kukushkin (KAZ) bt John Isner (USA x9) 6-4, 6-7 (3/7), 4-6, 6-1, 6-4
Matteo Berrettini (ITA x17) bt Marcos Baghdatis (CYP) 6-1, 7-6 (7/4), 6-3
Diego Schwartzman (ARG x24) bt Dominik Koepfer (GER) 6-0, 6-3, 7-5
Lucas Pouille (FRA x27) bt Grégoire Barrère (FRA) 6-1, 7-6 (7/0), 6-4
Roger Federer (SUI x2) bt Jay Clarke (GBR) 6-1, 7-6 (7/3), 6-2
Women
2nd round
Ashleigh Barty (AUS x1) bt Alison Van Uytvanck (BEL) 6-1, 6-3
Harriet Dart (GBR) bt Beatriz Haddad Maia (BRA) 7-6 (7/4), 3-6, 6-1
Alison Riske (USA) bt Ivana Jorovic (SRB) 6-2, 6-7 (3/7), 9-7
Belinda Bencic (SUI x13) bt Kaia Kanepi (EST) 6-3, 6-1
Serena Williams (USA x11) bt Kaja Juvan (SLO) 2-6, 6-2, 6-4
Julia Goerges (GER x18) bt Varvara Flink (RUS) 6-1, 6-4
Carla Suárez Navarro (ESP x30) bt Pauline Parmentier (FRA) 7-6 (7/2), 7-6 (7/4)
Lauren Davis (USA) bt Angelique Kerber (GER x5) 2-6, 6-2, 6-1
Kiki Bertens (NED x4) bt Taylor Townsend (USA) 3-6, 7-6 (7/5), 6-2
Barbora Strycova (CZE) bt Laura Siegemund (GER) 6-3, 7-5
Elise Mertens (BEL x21) bt Monica Niculescu (ROU) 7-5, 6-0
Wang Qiang (CHN x15) bt Tamara Zidansek (SLO) 6-1, 6-2
Sloane Stephens (USA x9) bt Wang Yafan (CHN) 6-0, 6-2
Johanna Konta (GBR x19) bt Katerina Siniaková (CZE) 6-3, 6-4
Magda Linette (POL) bt Amanda Anisimova (USA x25) 6-4, 7-5
Petra Kvitova (CZE x6) bt Kristina Mladenovic (FRA) 7-5, 6-2