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Brisbane - World No 2 Garbine Muguruza suffered a fitness scare ahead of this month's Australian Open when she was forced to retire from the Brisbane International on Tuesday with severe cramping.
Wimbledon champion Muguruza was ahead 2-1 in the deciding set against Serbia's Aleksandra Krunic in the second round of the warm-up tournament for the season's first Grand Slam event, when she collapsed to the ground following a serve.
The Spanish top seed was unable to continue and handed the match to Krunic 5-7, 7-6 (7/3), 2-1.
"I felt in trouble in the second set when I was 2-0 up," Muguruza said.
"I started to feel my calves were cramping."
Muguruza had won a tight first set and appeared heading for a straight sets win over Krunic when she opened up a 5-2 lead in the second.
However, she began to struggle in the 30 Celsius (86 Fahrenheit) heat and oppressive humidity as Krunic fought back to win the second set on a tiebreak.
Muguruza received treatment from the physio on court before the start of the decider and broke Krunic only to collapse while serving to consolidate the service break.
"I continued to think that with the match they (cramps) might go away, and then they were increasing, increasing. And then I had a lot of my body cramping," Muguruza said.
The loss ends any hope Muguruza had of leapfrogging Simona Halep and becoming world number one before the seedings are decided for the Australian Open, which begins on January 15.
Krunic will now play Anastasija Sevastova after the seventh-seeded Latvian crushed Sorana Cirstea of Romania 6-2, 6-1.
Last year's runner-up Alize Cornet eased into the final eight with a straight-sets win over Croatia's Mirjana Lucic-Baroni.
Cornet, who won her first-round match when fourth seed Caroline Garcia retired injured, was too consistent for Lucic-Baroni, winning 6-1, 7-5.
