Colombia fan (Getty Images)
The popular tabloid, known for its provocative headlines, ran with a front-page picture of England captain Harry Kane and the words "Go Kane!", a pun on Colombia's reputation as a drug exporter.
The sub-headline read "3 Lions face nation that gave world Shakira, great coffee and er, other stuff."
Nestor Osorio-Londono, Colombian ambassador to the UK, said it was "rather sad that they use such a festive and friendly environment, as the World Cup, to target a country and continue to stigmatise it with a completely unrelated issue".
"Respect, fair play and joy for the game is all that matters tonight. We'll be cheering for Colombia and hoping we can all enjoy a great match," he added.
The two nations face off against each other in Moscow on Tuesday for a place in the quarter-finals.