Cape Town - Kaizer Chiefs will face Maritzburg United in the Telkom Knockout Round of 16 while Orlando Pirates welcome Golden Arrows to Soweto.
Absa Premiership and defending Telkom champions Mamelodi Sundowns have been drawn against Polokwane City.
PSL newbies Cape Town City take on Bloemfontein Celtic while their arch-rivals, Ajax Cape Town, travel to Pretoria for a fixture against SuperSport United.
Meanwhile, Chippa United have been playing some of their best football this season and Highlands Park will visit the Eastern Cape side.
Dates and venues will be announced in due course.
Telkom Knockout fixtures:
Cape Town City v Bloemfontein Celtic
Baroka FC v Platinum Stars
Orlando Pirates v Golden Arrows
Free State Stars v Bidvest Wits
Kaizer Chiefs v Maritzburg United
SuperSport United v Ajax Cape Town
Mamelodi Sundowns v Polokwane City
Highlands Park v Chippa United