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Cape Town - Mpumalanga Black Aces and Bidvest Wits will battle for three Absa Premiership points when they meet at Mbombela Stadium.
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Aces saw their unbeaten run come to an end on the weekend when they were beaten 2-1 at home by Polokwane City.
The Mpumalanga club has claimed seven points from their six home matches this season.
Their next three league opponents are Chippa United, AmaZulu (both away) and Free State Stars (home).
AmaZayoni are placed ninth on the Absa Premiership log with 16 points from 11 matches.
Wits returned to winning form in the league with a 2-0 home win over Free State Stars on Saturday.
The Johannesburg team has claimed four points from five away matches this season.
Their next three league opponents are Kaizer Chiefs (home), Bloemfontein Celtic (away) and Ajax Cape Town (home).
The Clever Boys are placed sixth on the Absa Premiership log with 17 points from 11 matches.
In head-to-head stats, Aces and Wits have met in 31 league matches since 1985.
The Clever Boys have 16 wins compared to three for AmaZayoni, while 12 matches have been drawn.
Aces have won just two of their 16 home matches against Wits, suffering six defeats in the process.
Last season the Clever Boys took four points off AmaZayoni, drawing 0-0 in Johannesburg before winning the return game 2-1 in Mpumalanga.
Kick-off is at 19h30 on Wednesday, November 26.
