London - English Premiership sides will be on the look out for any potential "giant-killers" as they top 20 sides enter the competition in the third round of the prestigious knockout competition.
The second round ties will be played from December 5-8.
FA Cup second round draw:
Oxford United v Tranmere
Bury v Luton
Wrexham v Stevenage or Maidstone
Scunthorpe v Worcester
Colchester v Peterborough
Wycombe v AFC Wimbledon
Accrington v Yeovil
Hartlepool v Blyth Spartans
Milton Keynes Dons v Chesterfield
Southport v Eastleigh
Gateshead v Warrington
Oldham v Doncaster Rovers
Havant and Waterloovile or Preston North End v Shrewsbury
Barnsley v Chester
Bradford City v Dartford
Sheffield United v Plymouth Argyle
Cheltenham v Dover
Cambridge United v Mansfield
Bristol City v AFC Telford
Aldershot v Rochdale