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London - Mauricio Pochettino admitted Tottenham are suffering from fatigue after Michail Antonio fired West Ham to a 1-0 victory on Saturday, while Cardiff's 1-0 loss at Fulham leaves them in severe danger of relegation from the Premier League.
Pochettino's side had won all four matches at their new £1 billion Tottenham Hotspur Stadium.
But Antonio's clinical second half strike ended that run as the West Ham winger became the first visiting player to score in the plush arena and condemn Spurs to their 12th league loss of the season.
Third-placed Tottenham's second defeat in their last three Premier League games put a dent in their bid to finish in the top four.
They hold a three-point lead over fourth-placed Chelsea and are four points ahead of fifth placed Arsenal, with both teams in action on Sunday.
It was a frustrating warm-up for Tottenham's Champions League semi-final first leg against Ajax in north London on Tuesday.
Blaming Tottenham's hectic fixture list for their defeat, Pochettino said: "We cannot put away the circumstances. They arrived fresh and ready to compete, for us it is completely different.
"We were tired. We knew that it was going to be tough because it was a derby, but we have to move on.
"It was really tough today. We did not manage the ball properly. In the second half we conceded a lot of space for them to counter attack."
Conceding that Tottenham had hurt their hopes of qualifying for next season's Champions League, Pochettino added: "It is a fight, it is a race, with two games to play. We knew that before."
Third bottom Cardiff are four points from safety with just two games left after fourth bottom Brighton drew 1-1 with Newcastle.
Ayoze Perez fired Newcastle in front after 18 minutes, but Brighton improved after a woeful first half at the Amex Stadium.
Pascal Gross met Glenn Murray's flick to head the vital equaliser from close-range in the 75th minute.
Brighton's first goal in eight games in all competitions, a barren run of 735 minutes, sparked wild celebrations as the Seagulls edged closer to safety.
Lacklustre Cardiff
Cardiff had only themselves to blame for a lacklustre display against already relegated Fulham at Craven Cottage.
Former Liverpool forward Ryan Babel netted Fulham's winner when he took Cyrus Christie's pass and produced a superb finish from 25 yards in the 79th minute.
Neil Warnock's men almost snatched a draw in stoppage-time when Junior Hoilett side-footed against the bar.
"We've been kicked in the teeth up hill and down dale but I just told the players to keep going. They are trying the best they can," Warnock said.
Cardiff host Crystal Palace next weekend, while Brighton go to Arsenal.
Southampton sealed their survival in a dramatic 3-3 draw with south-coast rivals Bournemouth at St Mary's.
Shane Long scored the fastest goal in Premier League history after 7.69 seconds against Watford in midweek and the Irish forward struck again, this time after 12 minutes, to put Southampton ahead with a low shot.
Dan Gosling equalised for Bournemouth eight minutes later and the visitors were in front in the 32nd minute through Callum Wilson.
James Ward-Prowse bagged Southampton's equaliser from 20 yards in the 55th minute and Matt Targett made it three in the 67th minute, his effort ranking as the 1000th scored in the Premier League this season.
Wilson salvaged a point for Bournemouth with his 86th minute equaliser, but Southampton are assured of staying up.
Wolves gained a measure of revenge for their FA Cup semi-final defeat against Watford as they beat the Hornets 2-1 at Vicarage Road.
Mexico striker Raul Jimenez gave Wolves the lead in the 41st minute with a header from Diogo Jota's cross.
Andre Gray drew Watford level four minutes after half-time, but Jota won it for Wolves in the 77th minute as Nuno Espirito Santo's side consolidated seventh place.
Crystal Palace played out a dour goalless draw with Everton at Selhurst Park.
