London - Former All Black Charles Piutau and Ireland's Bundee Aki have been ruled out of the Barbarians' clash against England at Twickenham on Sunday.
New Zealand international Piutau suffered an Achilles tendon strain ahead of Ulster's European Champions Cup playoff victory against the Ospreys on Sunday.
And Ireland centre Aki, who was due to captain the BaaBaas against England, is nursing an ankle injury.
The Barbarians also announced that Sale Sharks flyhalf AJ MacGinty has been withdrawn from the squad by his club.
BaaBaas head coach Pat Lam has now finalised his group for the England encounter, adding five more players in Scotland flyhalf Finn Russell, New Zealand centre Malakai Fekitoa, Newcastle and Fiji centre Josh Matavesi, his Falcons colleague, flank Nili Latu, and Connacht prop Denis Buckley.
"It's unfortunate that Charles Piutau strained an Achilles tendon warming up for Ulster on the weekend, Bundee Aki rolled an ankle in training in Galway and AJ MacGinty has been pulled out by his club," said Lam.
"But it's great that we are able to call in some more quality players."
The Barbarians starting line-up is due to be announced on Thursday.
Revised Barbarians 23-man squad:
Niyi Adeolokun (Connacht & Ireland)
Chris Ashton (Toulon & England)
Malakai Fekitoa (Toulon & New
Greig Laidlaw (Clermont &
Luke McAlister (Toulon & New
Josh Matavesi (Newcastle Falcons
Semi Radradra (Toulon)
Finn Russell (Glasgow Warriors &
Josua Tuisova (Toulon & Fiji)
Rhodri Williams (Bristol &
John Afoa (Gloucester & New
Denis Buckley (Connacht)
Ultan Dillane (Connacht &
Juan Martin Fernandez Lobbe (Toulon
Ramiro Herrera (Stade Francais &
Benjamin Kayser (Clermont &
Nili Latu (Newcastle Falcons &
Tatafu Polota-Nau (Leicester &
Sateleki Timani (Clermont &
Justin Tipuric (Ospreys & Wales)
Loni Uhila (Clermont)
Flip van der Merwe (Clermont &
Victor Vito (La Rochelle & New