Cape Town - The Springboks will be without their overseas-based players for the June 2 Test against Wales in Washington DC.
This comes after three England-based Welsh players - Luke Charteris, Tomas Francis and Josh Adams - were withdrawn from the squad for that match because the Test falls outside World Rugby international Test window.
Even though the English season ends on May 26, clubs can still be fined if they allow their players play for their countries outside of an international window.
It means that Rassie Erasmus will be without the likes of Faf de Klerk and Willie le Roux for the Wales Test, with both of those players having been tipped to make a return to the national set-up.
A squad of 26 locally-based Super Rugby players will represent the Boks against Wales, while the overseas-based players will come into the squad for the three-Test series against England, starting on June 9.