Cape Town - Italy head coach Conor O'Shea has named Michele Campagnaro and Tommaso Allan in his backline that will take on England on Sunday.
They are two of three changes in the backs as Giulio Bisegni also comes in on the wing, with Tommaso Benvenuti and Carlo Canna dropping to bench duty due to Campagnaro and Allan's promotion to the starting team.
Angelo Esposito drops out of the squad altogether while the only other change to the side that lost to Ireland sees Abraham Steyn replace Maxime Mata Mbanda, who will be on the replacements bench at Twickenham.
Italy have lost both of their fixtures thus far in the Six Nations, conceding a total of 96 points which includes 12 tries in the process.
15 Edoardo Padovani, 14 Giulio Bisegni, 13 Michele Campagnaro, 12 Luke McLean, 11 Giovanbattista Venditti, 10 Tommaso Allan, 9 Edoardo Gori, 8 Sergio Parisse (c), 7 Simone Favaro, 6 Abraham Steyn, 5 Andries Van Schalkwyk, 4 Marco Fuser, 3 Lorenzo Cittadini, 2 Leonardo Ghiraldini, 1 Andrea Lovotti
Substitutes: 16 Ornel Gega, 17 Michele Rizzo, 18 Pietro Ceccarelli, 19 George Biagi, 20 Maxime Mata Mbanda', 21 Giorgio Bronzini, 22 Carlo Canna, 23 Tommaso Benvenuti