According to the Lions' website, flyhalf Marnitz Boshoff has fractured his right arm and will be out of action for up to 12 weeks. He will undergo surgery on Monday morning.
In addition, both Anthony Volmink (shoulder) and Lloyd Greeff (knee) are also expected to be out of action for the remainder of the Currie Cup tournament.
Other members of the Golden Lions backline Alwyn Hollenbach (shoulder), Michael Bondesio (knee), Courtnall Skosan (cheek bone) and Deon van Rensburg (ribs) are all recovering well and are expected to return to action in the upcoming weeks.
Meanwhile, in the forwards, Julian Redelinghuys (calf) is also expected to be available for selection again soon.
The Golden Lions' next match is against the EP Kings in Johannesburg on Saturday. Kick-off is at 14:30.