Gatlin, who was beaten for the world title by Usain Bolt in Moscow earlier this month, clocked 10.08 seconds to beat Ashmeade by 0.09. Keston Bledmann of Trinidad and Tobago trailed Gatlin by 0.19 in third.
World record-holder Aries Merritt of the United States beat 2008 Olympic champion Dayron Robles by 0.23 to take the 110m hurdles in 13.26, while Olympic champion Sally Pearson of Australia won the women's 100m hurdles in 12.83, leading second-place Reina-Flor Okori of France by 0.35.
Barbara Pierre won the women's 100m ahead of Charonda Williams and Alexandra Anderson to lead an American sweep of the podium.
Results from the Gugl Games at the Linzer Stadium:Men:
100m:
1. Justin Gatlin, United States, 10.08 seconds.
2. Nickel Ashmeade, Jamaica, 10.17.
3. Keston Bledmann, Trinidad and Tobago, 10.27.
4. Jeff Demps, United States, 10.29.
5. Ryan Bailey, United States, 10.35.
6. Calesio Newman, United States, 10.44.
7. Gabriel Mvumvure, Zimbabwe, 10.48.
8. Harry Aikines-Aryeetey, Britain, 10.49.
9. Mark Lewis-Francis, Britain, 10.59.
110m hurdles:
1. Aries Merritt, United States, 13.26.
2. Dayron Robles, Cuba, 13.49.
3. Mikel Thomas, Trinidad and Tobago, 13.51.
4. Joel Brown, United States, 13.76.
5. Gregor Traber, Germany, 13.77.
6. Ray Stewart, United States, 14.07.
7. Manuel Prazak, Austria, 14.36.
8. Martin Kainrath, Austria, 15.02.
Women:
100m:
1. Barbara Pierre, United States, 11.14.
2. Charonda Williams, United States, 11.23.
3. Alexandra Anderson, United States, 11,27.
4. Lekeisha Lawson, United States, 11,31.
5. Batalia Pohrebnyak, Russia, 11.38.
6. Schillonie Calvert, Jamaica, 11.49.
7. Aleen Bailey, Jamaica, 11.64.
8. Carina Horn, Austria, 11.90.
100m hurdles:
1. Sally Pearson, Australia, 12.82.
2. Reina-Flor Okori, France, 13.17.
3. Nadine Hildebrand, Germany, 13.20. tie, Kellie Wells, United States, 13.20.
5. Jackie Coward, United States, 13.27.
6. Andrea Bliss, Jamaica, 13.28.
7. Lucie Skrobakova, Slovakia 13.37.
8. Ayako Kimura, Japan, 13.57.
9. Linda Thoms, Austria, 14.80.
400 hurdles:
1. Dalilah Muhammad, United States, 55.01.
2. Denisa Rosolova, Czech Republic, 55.27.
3. Irina Davydova, Russia, 56.53.
4. Christine Spence, United States, 57.49.
5. Axelle Dauwens, Belgium, 57.83.
6. Manuela Gentili, Italy, 57.96.
7. Anastasia Ott, Russia, 58.61.
8. Jana Reissova, Czech Republic, 62,59.