Perth - India suffered a big setback on the eve of the second Test against Australia when ace spinner Ravichandran Ashwin and experienced batsman Rohit Sharma were ruled out on Thursday.
Also missing, as expected, will be young opener Prithvi Shaw, who was not considered for the opening Test in Adelaide after suffering ligament damage in a warm-up match.
"Ravi Ashwin has a left-sided abdominal strain. He is receiving treatment at the moment. He has been ruled out of the second Test," India's governing body, the BCCI, said.
"Rohit Sharma jarred his lower back while fielding in the first Test at Adelaide. He is undergoing treatment, he too is ruled out of the second Test."
The BCCI added that Shaw was "recovering well" but still undergoing treatment.
Ashwin took six wickets in the first Test, which India won by 31 runs, while Sharma hit 37 and one.
India named a 13-man team for the Test starting on Friday, which is set to be played on a fast and bouncy Perth Stadium wicket.
All-rounder Hanuma Vihari, who can bowl off-spin, was included, as was Ravindra Jadeja who has taken 185 Test wickets with his left-arm spin and also averages 32.44 with the bat in 39 appearances for India.
Virat Kohli (captain), Murali Vijay, KL Rahul, Cheteshwar Pujara, Ajinkya Rahane, Hanuma Vihari, Rishabh Pant, Ravindra Jadeja, Ishant Sharma, Mohammed Shami, Jasprit Bumrah, Bhuvneshwar Kumar, Umesh Yadav