He will open the batting for the KwaZulu-Natal amateur team in a limited-overs match against South-Western Districts at the Chatsworth Oval on Sunday.
Amla, who replaced the injured Graeme Smith in the one-day series in England, suffered the injury during the fourth limited-overs international at the end of August and has been out of action since then, including missing the opening three SuperSport Series matches for the Dolphins.
"On the advice of the physiotherapist, I have been strapping the ankle during practice sessions. The treatment has been working well and the ankle is almost 100 percent. All I need now is to play in a match situation to give it a good run out because I've been out of action for a while. Therefore I decided to play at Chatsworth on Sunday," he told SuperCricket.
Amla will then travel to Kimberley on Monday, where he will join his national teammates for a training camp ahead of the two one-day internationals against Kenya. The first ODI takes place in Bloemfontein on October 31 and the second in Kimberley on November 2.
The Proteas then host Bangladesh, before touring Australia in December.