Eastbourne - Petra Kvitova will head to next week's start of her Wimbledon title defence without a win on grass after exiting in her opening match at the Eastbourne event on Tuesday 7-5, 6-4 to Ekaterina Makarova.
Czech Kvitova was humbled at Devonshire park in 91 minutes in the second round by the Russian, who benefitted from six Kvitova double-faults and broke the second seed five times.
On the men's side, Belgian Steve Darcis put out Australian Matthew Ebden 6-2, 6-4 while Uzbek Denis Istomin beat Frenchman Michael Llodra 6-4, 6-3.
The Makarova win was a reversal of a victory here a year ago by Kvitova over the Russian in the second round.
The 48th-ranked Makarova, winner of the 2010 Eastbourne title, gained grass experience last week in Birmingham as she reached the semi-finals.
Results on Tuesday from the Aegon International, a combined WTA and ATP grass-court event at Devonshire Park Lawn Tennis Club (seedings in parentheses):
Steve Darcis, Belgium, def. Matthew Ebden, Australia, 6-2, 6-4.
Denis Istomin, Uzbekistan, def. Michael Llodra, France, 6-4, 6-3.
Lu Yen-hsun, Taiwan, def. Julien Benneteau (5), France, 6-3, 2-6, 7-6 (7/4).
Andy Roddick (6), United States, def. Sam Querrey, United States, 5-2, retired.
Philipp Kohlschreiber (7), Germany, def. Go Soeda, Japan, 6-4, 7-5.
Marinko Matosevic, Australia, def. Richard Gasquet (1), France, 1-6, 7-6 (7/5), 7-6 (7/3).
Ekaterina Makarova, Russia, def. Petra Kvitova (2), Czech Republic, 7-5, 6-4.
Angelique Kerber (5), Germany, def. Elena Vesnina, Russia, 3-6, 6-0, 7-5.
Klara Zakopalova, Czech Republic, def. Zheng Jie, China, 6-3, 4-6, 6-3.
Tsvetana Pironkova, Bulgaria, def. Agnieszka Radwanska (1), Poland, 6-2, 6-4.
Christina McHale, United States, def. Caroline Wozniacki (3), Denmark, 6-1, 6-7 (7/9), 6-4
Marion Bartoli (4), France, def. Sorana Cirstea, Romania, 6-2, 6-2
Laura Robson, Britain, def. Maria Jose Martinez Sanchez, Spain, 3-6, 6-2, 6-2
Anastasia Pavlyuchenkova, Russia, def. Hsieh Su-wei, Taiwan, 6-2, 3-0, retired.
Heather Watson, Britain, def. Greta Arn, Hungary, 6-3, 6-4.
Petra Cetkovska (9), Czech Republic, def. Andrea Hlavackova, Czech Republic, 3-6, 6-2, 6-1.
Lucie Safarova (7), Czech Republic, def. Timea Babos, Hungary, 3-6, 6-3, 7-6 (8/6).
Stephanie Dubois, Canada, def. Carla Suarez Navarro, Spain, 6-4, 6-4.