The former Golden Arrows midfielder, who found himself way down the pecking order at the beginning of the season, was handed a life line by the coach to train with the first team.
The playmaker managed to impress the Dutch tactician and was handed the chance to play in several friendly matches; however, the midfielder has since decided against joining the Brazilians and has opted out of his trials.
"No not any more," Neeskens told Football365.co.za when asked whether there is the possibility of seeing the former Bafana Bafana ace in action again in Tshwane.
"That is something between the club, player and the coach. We gave him the opportunity and unfortunately after one-and-half-weeks he SMSed myself and said he does not want to be with the team anymore, so that was his decision," he said.
Neeskens went on to reveal he has been left disappointed by Zothwane's decision as the Downs boss felt the centre-mid could bring a different dynamic to the squad.
"He didn't want to practice any more; he wanted to depart so it is his decision. So for me we gave him the chance that he could come back but he decided not to fight for it, he said he does not want to be with us any more. He prefers to practice aside and that his decision and we have to respect that," he said.
"He is no longer with us, not training with the reserves, he now trains alone. So it is between him and club and we wish him all the best. We wanted to give him more chance but it's his decision we respect it," the coach concluded.