Kick-off is at 7:30pm.
'Downs recorded a ninth successive league win when they defeated Moroka Swallows 1-0 in their last outing.
SuperSport United have not won any of their last three league matches, losing 1-0 to Golden Arrows last time out.
Former United midfielder Teko Modise, who has been a vital cog in 'Downs success is expecting an emotional game on Tuesday as the Chloorkop based side look to wrap up the league title.
“It's one of those games where it's going to be very emotional because we know the importance of it,” Modise said ahead of the encounter.
“We know what can happen if we win it or what can happen if we lose it. We're just one game away from realising our dream and hopefully we'll be able to do that.”
Matsatsantsa assistant coach Kwanele Kopo has explained that United will be aiming to keep Pitso Mosimane's side quiet for as long as possible as they look to spoil 'Downs party.
“The stakes are very high, Sundowns want to clinch the league title and we need to take care of our own position on the log and make sure we secure a top eight finish,” said Kopo.
“It is going to be a very difficult game, they will throw everything at us and try and wrap up the title in front of their fans. But we have come up with a tactical approach that we are confident in and hopefully we can keep the tempo down, keep them quiet for as long as possible and surprise them.”
Since 1990, Sundowns have faced SuperSport in 45 league matches. The Brazilians edge the victories by 20 wins to Matsatsantsa's 14, while 11 matches have been drawn.
When the teams met earlier this season they played to a 1-1 draw in a home match for Matsatsantsa.
Lebohang Manyama is ruled out for the visitors while Bevan Fransman, Mor Diouf and Bradley Grobler will all face a fitness test.
Mosimane meanwhile can recall on the services of Alje Schut, Suprise Moriri, Wayne Sandilands and Mzikayise Mashaba.