Cape Town - AmaZulu captain Robyn Johannes was reportedly involved in a bust-up with team-mate Mbulelo Mabizela after their 3-1 defeat to Supersport United on Saturday night.
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Mabizela had a poor game in Durban and was at fault for Matsatsantsa's first two goals, scored by Daine Klate and Jeremy Brockie either side of half-time.
The 34-year-old defender capped off a miserable evening when he went into the referee's book, which was his fourth yellow card of the season and resulted in an automatic one-match ban.
The loss at the Moses Mabhida Stadium left Usuthu rooted to the foot of the Premiership table, three points adrift of 15th-placed Moroka Swallows with just two games left to play, meaning they face at least a relegation play-off battle at the end of the season.
According to reports, Johannes and Mabizela clashed as the players were heading towards the dressing room after the final whistle, with the club's skipper launching a water bottle at 'OJ' in retaliation to being shoved by his defensive partner.
The pair apparently had to be separated as goalkeeper Sedat Ouro-Akoriko stepped in to pull Johannes away from the fray.
Usuthu marketing manager, Lunga Sokhela, is reported to have called Steve Barker's squad into the dressing room afterwards, where an hour-long meeting followed.