Nelspruit - The 18-member SA women's football squad announced last week will remain unchanged for next month's London Olympic Games.
Banyana Banyana coach Joseph Mkhonza had hoped the key injured due of Jabulile Ntsibande and Yola Jafta would recover in time for the deadline to make changes at the end of June.
"Unfortunately, we've just received news that they won't be able to make it," Mkhonza said on Wednesday.
"Even if they do recover, they will still need another six weeks [to prepare].
"We thought maybe they would recover and were going to give them a chance.
"They won't be part of team, so the squad announced will stay the same."
The side's captain, Amanda Dlamini, has shaken off a knee injury and proclaimed herself fully fit for camp in the build-up to Banyana's Olympic debut, while star defender Janine van Wyk was expected to recover from a groin injury ahead of the tournament.
Van Wyk is not part of the squad preparing for the African Women's Championship return-leg qualifier against Zambia at in Nelspruit on Saturday.
The South Africans carry a 4-1 lead from the first leg, heading into their final match at home before the global showpiece.
"We have rested [Van Wyk] as we were advised by the medical team," Mkhonza said.
"In two weeks' time she'll be fine. She came here [to camp] and we said let's leave her out to recover."
Banyana have lined up an international fixture against Nigeria in Lagos next weekend.
The players will then return to feature for their respective clubs in the Sasol National Championships.
Their final pre-Olympic camp will be held in Germany in early July.
While Mkhonza raised a concern of possible injuries, he felt it was important for the players to partake in the domestic women's club championships ahead of their last camp.
"We are hoping that we don't get any more injuries for the tournament, because after that we have Germany," he said.
"But we have got players from these clubs so we have to respect the teams because they also need them."