Signed by the defending champions on a short-term contract last week due to their backline injury crisis, Lane becomes the Reds' fifth-choice flyhalf this season after being chosen to start ahead of Dallan Murphy at Suncorp Stadium.
The 21-year-old son of former Wallabies centre Tim Lane was a boom prospect in South Africa, where he finished his schooling in Cape Town, before returning to Sydney late last year to take a spot in Australia's national academy.
Lane is one of four personnel changes Reds coach Ewen McKenzie has made to the starting team which lost 45-19 to the Force last weekend in Perth.
Speedy outside back Aidan Toua will make his run-on debut at outside centre after overcoming a hamstring strain sustained in the pre-season while lock Rob Simmons and flanker Beau Robinson return to the starting pack.
McKenzie has opted for two ball scavengers in a mobile back-row with Robinson and Liam Gill to work in tandem against the Brumbies' big loose-forward trio of Ben Mowen, Peter Kimlin and Ita Vaea.
With Scott Higginbotham moving to the back of the scrum, Radike Samo has again been overlooked at No 8 and will make his return off the bench as one of five Reds forwards.
McKenzie has also switched Luke Morahan back to fullback with Rod Davies to start on the wing.
The Brumbies, who arrived in Brisbane on Thursday morning, hold a five-point lead at the top of the Australian conference and can open up a sizeable gap on the Reds if they dish out the Reds fourth straight defeat.
Teams:
Reds:
Substitutes: 16 Albert Anae, 17 Greg Holmes, 18 Radike Samo, 19 Van Humphries, 20 Eddie Quirk, 21 Nick Frisby, 22 Dallan Murphy
Brumbies:
15 Jesse Mogg, 14 Joe Tomane, 13 Andrew Smith, 12 Pat McCabe, 11 Henry Speight, 0 Christian Lealiifano, 9 Nic White, 8 Ben Mowen (captain), 7 Ita Vaea, 6 Peter Kimlin, 5 Leon Power, 4 Sam Carter, 3 Dan Palmer, 2 Stephen Moore, 1 Ben Alexander
Substitutes: 16 Anthony Hegarty, 17 Ruaidhri Murphy, 18 Scott Fardy, 19 Michael Hooper, 20 Ian Prior, 21 Zack Holmes, 22 Tevita Kuridrani