There were concerns that the fight would not take place after Bruwer refused to be weighed at the pre-fight medicals on Tuesday.
A confident Bruwer, however, tipped the scale at 90.86 kilograms on Friday, with Simba weighing in at 90.26.
"I'm confident because Flo doesn't have the experience," Bruwer said.
"I'm not underestimating him, but I look at the guys that he has knocked out and none of them are big names, so I will handle him."
The bout was turned to a catchweight fight with neither pugilist making the South African 86kg limit for the cruiserweight division.
Simba, a former heavyweight prospect, lost seven kilograms, and looked in good physical shape ahead of the fight which could decide his future in the ring.
He admitted there was pressure from his family to bow out of the sport if he lost.
"It is an option. My family wants me to concentrate on my studies and consider something more 'normal'," said Simba, who is studying civil engineering.
"I am looking forward to this [fight with Bruwer] and the Super 8 tournament [in June]."
Should the match be decided on points after 10 rounds, both fighters will enter the Super 8 contest.
If either gets knocked out, though, they will make way for Ruben Groenewald to enter the competition.
In the main fight on Saturday's Golden Gloves bill, South African Tommy Oosthuizen will defend his IBO super-middleweight title against Serge Yannick from Australia.
Kaizer Mabuza meets Steve Wills, also from Australia, for the vacant IBO junior-middleweight belt.