GALLERY: Pistorius at the Olympics
Pistorius finished last in his semi-final heat on Sunday evening, coming home in a time of 46.54 seconds.
Grenada's Kirani James, last year's world champion, won this second semi-final heat in 44.59 seconds.
Pistorius on Saturday made history as the first amputee to participate in a track event at the Olympic Games.
He bowed out, waving to the crowd in gratitude for their support.
In a great sporting gesture, James immediately walked over to Pistorius after the race and asked for his name bib as a souvenir.
Pistorius is still expected to run the 4x400 relays, which start on Thursday. Last year, Pistorius and South Africa won the silver at the world championships.