England coach Andy Flower criticised the International Cricket Council on Friday after South Africa captain Graeme Smith survived a review from third umpire Daryl Harper following an appeal for caught behind.
"The ECB accepts that the decision must remain concerning the dismissal, but has issued a formal appeal following play on day two and contends that the lost referral must be reinstated," the ECB statement said.
The ECB statement quoted chairperson Giles Clarke as saying: "We have grave concerns about how this process was implemented and I will be addressing the issue at the highest levels within ICC during this match."
England were convinced Smith had edged the ball and Flower said Harper had erred by not turning up the volume on his feed from the stump microphone, adding that the England camp could clearly hear an edge on the television sets in their dressing room.
Smith, on 15 at the time, went on to make 105 as South Africa took a stranglehold on a test they need to win to square the four-match series.
The ICC had earlier issued a statement saying Flower was incorrect in saying the volume had not been turned up on Harper's television feed.
"There have also been suggestions...that Mr Harper had turned down the feed volume," the ICC said.
"It is clarified that the volume on the third umpire's feed, right throughout the series, had been configured to optimise the quality of the audio, by both an SABC [South African Broadcasting Corporation] Head Engineer and the ICC technical advisor."